Chromatography Lab
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by Liz LaRosa
www.middleschoolscience.com
click
here for a modified pdf version
Objectives:
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To separate pigments found in markers. (Rose Art works the
best. Do not use Crayola- won't separate)
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To determine the primary colors of pigments.
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To calculate the Rf value of primary colors.
Materials:
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Filter paper
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Water
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Beaker
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Markers (3 per group)
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Pencil
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Ruler
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Calculator
Procedure:
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Cut round filter paper in half. Cut off one end to make a
flat edge. ( will look like a sailboat sail)
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Measure 3cm from the bottom and draw a line across the width
in pencil.
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Each group will have 3 markers: a primary color (red, yellow
or blue) a secondary color ( orange, green, purple) and the last color
is black or brown.
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On the starting line, dab one color at a time, about 10 times
each. The 3 colors should not touch. You will have 3 dots on the starting
line.
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In a beaker, add a small amount of water about 1 - 2 mL.
The water should not
touch the pigments!!!
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Place paper in beaker flat edge down and let sit 10 - 15
minutes.
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Take out paper and, with your pencil, follow the water line
across to mark the boundary between wet and dry. This is your finish line.
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With your pencil, circle each pigment and label. (i.e. b
for blue)
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Measure each of the 3 primary colors in cm from the starting
line to where the pigment ended. Record in Table 1.
Data :
Table 1 - Observations of pigments
(half page)
Color
|
Distance Pigment cm
|
Distance Water Traveled cm
|
Rf Value = Pigment/Water
|
Red
|
|
|
|
Yellow
|
|
|
|
Blue
|
|
|
|
Analysis/Results:
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Which color had the highest Rf value?
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Rank the colors from highest to lowest.
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Compare your results with the class.
What were their Rf values? Do you notice any trends? Explain.
Conclusion:
2-3 sentences on what you learned
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